Biosynthesis of L-lysine and L-threonine in Brevibacterium. III. Production of L-lysine by leucine auxotrophs derived from AEC resistant mutant of Brevibacterium lactofermentum.

Microbial Production of L-lysine I. Production by Auxotropiis of Brevibacterium Elavum

Seven homoserine, 42 methionine, 42 threonine, and 15 isoleucine auxotrophs were obtained from Brevibacterium flavum No. 2247 (ATCC No. 14067) by X-ray irradiation and N-methyl-N'-nitro-N-nitrosoguanidine treatment. Concerning the L-lysine-producing ability, the homoserine auxotrophs were the best and about one-half of the threonine auxotrophs ranked next. The rest of threonine auxotrophs and a...

Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase

5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It is a metabolite of l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. L-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA) play key roles in the biotransformation of L-lysine into 5-aminovalerate. Here, DavB and D...

Cloning and characterization of genes responsible for m-fluoro-D,L-phenylalanine resistance in Brevibacterium lactofermentum.

Two kinds of 3-deoxy-D-arabino-hepturosonate-7-phosphate (DAHP) synthase genes were cloned from an L-Phe-producing mutant of Brevibacterium lactofermentum, AJ11957, which was resistant to m-fluoro-D,L-phenylalanine (mFP) and p-fluoro-D,L-phenylalanine (pFP) and which had DAHP synthase free from feedback inhibition. Both genes were cloned using a vector plasmid, pAJ1844, and the resulting recomb...

Enhanced production of L-lysine from Corynebacterium glutamicum by mutagenesis

Influence of Oxygen and pH on Methanethiol Production from l-Methionine by Brevibacterium linens CNRZ 918.

The effects of dissolved oxygen concentration and pH on the growth of Brevibacterium linens CNRZ 918 and its production of methanethiol from l-methionine were investigated. Optimal specific methanethiol production was obtained at 25% saturation of dissolved oxygen and at a pH between 8 and 9, whereas optimal cell growth occurred at 50% oxygen saturation and when the pH was maintained constantly...